Description of Human Liver Microtissues
A turnkey screening solution for identifying Hepatotoxic compounds. 3D InSight™ Human Liver Microtissues is the gold standard for toxicity testing. These pharma-validated, preclinical co-culture liver models have been shown to achieve 2x higher sensitivity for DILI prediction compared to traditional 2D hepatocytes.
By combining multi-donor diversity with structural and functional robustness, our models are ideal for drug safety and efficacy testing as well as the study of healthy and diseased liver function.
Microtissues are shipped in our InFloat™ Shipping System which includes our Akura™ 96 plates (one microtissue per well), with 3D InSight™ Human Liver Microtissue Maintenance Medium - TOX/AF/INF, our recommended medium for routine culture and maintenance of liver microtissues.
Learn more about the product and access resources:
Advantages of 96 Well Plates & Microtissues
- Reflect the average response of a population with a model incorporating hepatocytes from 5 male and 5 female donors
- Achieve the highest biological relevance in an in vitro model with primary hepatocyte, Kupffer cell, and liver endothelial cell (LEC) co-cultures
- Test long-term effects of drug exposure in an organotypic, functional liver model
- Highly reproducible and robust liquid handling in InSphero’s patented Akura™ 96 plates (engineered to protect microtissues from accidental aspiration)
- Complete turnkey solutions offer lower cost-of-ownership than in-house production
Technical Specifications
- PHH from pooled male/female donors
- Co-culture with Kupffer cells and LECs
- Guaranteed 28 days viability
- 96-well format, 1 microtissue per well
- Delivered with 250 ML of 3D InSight™ Liver Maintenance Medium TOX/AF
- Biweekly production. Check our online production schedule for details
- Shipping date to be confirmed by our customer service team after checkout
Human Liver Microtissues FAQs
Q: When do I get my Microtissues?
A: We produce 3D InSight™ Human Liver Microtissues every two weeks. (Check the Liver Microtissue Production Schedule for upcoming production runs.) After your order is completed, our customer service team will contact you by email to notify you of the next shipping dates.
Q: How do you ship?
A: We ship our human liver microtissues in a patent-pending shipping container that maintains the microtissue functionality and integrity perfectly. The transport method depends on the destination country.
Typically, the human liver microtissues ship via companies such as Fedex, UPS, and DHL or direct commercial airline flights such as Swiss Air. When you place your order, please choose an appropriate ship-to address and contact name so the microtissues will be delivered promptly to your lab.
Q: Where are the 3D Human Liver Microtissues produced?
A: The microtissues are produced in our production facility in Schlieren, Switzerland outside of Zurich.
Q: What is the difference between the variants TOX, INF, and AF media?
A: These are different variants of Human Liver Maintenance Medium, all serum-free and designed to support safety assessments.
3D InSight™ TOX Liver Medium
- Purpose: Standard medium supporting phase I metabolism for small molecule safety assessments for 4 weeks of culture.
- Characteristics: Contains dexamethasone, high glucose, and high insulin.
3D InSight™ INF Liver Medium
- Purpose: Optimized for enhanced immuno-competency, though with reduced phase I metabolism after extended culture time.
- Characteristics: Same base as TOX medium (high glucose and high insulin), but without dexamethasone.
3D InSight™ AF (Advanced Formula)
- Purpose: Primarily for contingency purposes, replaced in standard services by TOX Liver medium
Q: Will I receive a shipment tracking number?
A: If the microtissues ship via Fedex, UPS, or DHL you will receive a tracking number. If the shipment travels via a direct commercial flight you will receive a notification of departure and scheduled arrival date. InSphero monitors the shipment until delivery.
Q: What should I do with the Microtissues upon arrival?
A: Open the outer box and retrieve the temperature logger from the internal microtissue box. Press Start/Stop button on the monitor to view confirmation that the appropriate temperature was maintained during transport. Change the media upon arrival or place the microtissues in a 37⁰C incubator until you can change the media.
Follow the instructions in the Quick Start Guide for microtissue maintenance upon arrival. Additional instructions can be found in InSphero’s microtissue handling guide.
Q: What toxicology studies and assays can I run with my human liver microtissues?
A: See InSphero’s website with this link for examples of hepatotoxicity data generated reports with InSphero’s 3D liver microtissues.
Q: Why should I use InSphero's 3D InSight™ Microtissues?
A: Our standardized, scaffold-free liver microtissues are engineered and manufactured to the highest quality specifications using our 3D Select process in state-of-the-art facilities by our staff of 3D experts in toxicology and metabolic diseases.
Receiving our assay-ready, user-friendly format means you can focus your resources on generating more relevant data in 3D models.
InSphero Sales Terms and Conditions
Prior to placing an order on this page, we recommend that you first read the InSphero Terms and Conditions. This document includes our disclaimer, imprint, and detailed terms and conditions information.
Shipping Human Liver Microtissues
Curious how we ship live, assay-ready 3D microtissues to labs in most regions?
Shipping costs will be calculated based on the quantity of the plates ordered and the shipping destination. Our service team will contact you to review order details and verify ship dates prior to confirming your order.
Learn more about our microtissue shipping technology and view our unpacking 3D InSight™ Microtissues documentation.