The Akura™ 3D SureKit provides a validated all-in-one solution to generate and culture organotypic 3D spheroids from primary human hepatocytes.
The 3D SureKit contains frozen primary human hepatocytes and specialized media to aggregate the cells and culture-formed spheroids. 3D optimized Akura™ 96 plates are included to allow for homogenous aggregation and loss-free media exchanges. Reference characterization data and dose-response curves for model DILI compounds are provided as benchmark data for your in-house experiments.
- Hepatocytes guaranteed to form 3D spheroids
- The best-in-class 3D cell culture compatible Akura™ 96 Spheroid Microplates, which guarantee homogenous aggregation of cells, minimal tissue loss during media exchanges, and an optimized geometry designed to minimize media evaporation
- InSphero proprietary aggregation and culture media are essential for generating and maintaining spheroids
- Access to QC data and protocols
Catalog-No.: KT-05-002-01
Note: This Akura™ 3D SureKit contains validated hepatocytes from a single donor. If you would like to work with more complex models or microtissues from pooled donors, please check our 3D InSight™ products or talk to our customer service representative.
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Components of the Akura™ 3D SureKit
- 3 x Akura™ 96 Spheroid Microplates
- 1 x Akura™ Tilting Stand
- Primary human hepatocytes, cryopreserved (> 1 million per vial)
- 40 ml Aggregation base medium stored at 4°C
- 12.5 ml Aggregation supplement stored at -20°C
- 200 ml Maintenance base medium stored at 4°C
- 10 ml Maintenance supplement stored at -20°C